egfr 18-21 mastr research application C for detection of somatic variants, snvs, in exons 18-21 of egfr in ffpe-derived dna the egfr 18-21 mastr is a molecular research panel for the identification of variants in exons 18 to 21 of the egfr gene, which codes for the epidermal growth factor receptor (egfr). this assay is ready to use and offers robust performance with minimum hands-on time. it consists of all the reagents necessary to enable multiplex amplification of 4 amplicons for complete coverage of exons 18 to 21 of the egfr gene. performance dna input as low as 4 ng per plex reaction number of samples/run (20 reads/allele): @ 5 % vaf sample illumina miseq v2: 2,057* illumina miseq v3: 3,771* assay characteristics genes analyzed full coding region of exons 18-21 of egfr genomic region analyzed 0.6 kb number of amplicons 4 amplicon length 100-180 bp number of plexes 1 designed to be compatible with illumina miseq, miniseq, nextseq *only 192 mid combinations available.
www.agilent.com not for eu genomics@agilent.com for research use only. not for use in diagnostic procedures. this information is subject to change without notice. pr7000-0726 ? agilent technologies, inc. 2017 published in the usa, october, 2017 5991-8370enuc ordering information cat. no. product name samples mr-0130.024 egfr 18-21 mastr 24 mid (molecular identifiers) kits are necessary to complete the workflow mastr workflow plan run multiplex pcr quality control universal pcr quality control multiplex pcr dilution universal pcr equimolar pooling mixing plexes of single sample purifcation mixing clean up pooling sequencing data analysis ngs
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